Biomolecular Interaction Analysis

BIAffinity®  offers a technique for label-free detection of biomolecular interactions on solid phases, i. e., biochips. The system’s most impressive features are its intuitive and simple handling, flexible sample volumes, wide choice of functional chip surfaces and self-explanatory software for data collection and evaluation. The system permits direct, time-resolved detection of binding events on specific surfaces and enables access to thermodynamic and kinetic characterizations of affinity reactions.

 

Fol_BIAffinity_e

BIAffinity®  offers a technique for label-free detection of biomolecular interactions on solid phases, i. e., biochips.

The system’s most impressive features are its intuitive and simple handling, flexible sample volumes, wide choice of functional chip surfaces and self-explanatory software for data collection and evaluation.

The system permits direct, time-resolved detection of binding events on specific surfaces and enables access to thermodynamic and kinetic characterizations of affinity reactions.

 

 

 

 

 
  • Label-free detection of molecular interaction
  • Real-time analysis
  • Biochip technology
  • Reflectometric interference spectroscopy (RiFS)
  • Determination of kinetic and equilibrium constants and concentration
  • Referencing against non-specific interactions
  • Two-channel flow system
  • High-performance two channel optics
  • Temperature-controlled operation environment
  • User administration
  • Available with BIAsampler (automared 96 well autosampler)

 

High performance fluidics...

...is an important element of BIAffinity® .The two-channel microfluidic cell (MFC) supplies the sensor surface with a continuous and pulse-free liquid low. A measurement and reference channel are available for each measurement, which allows referencing against non-specific interactions on the surface. Rapid exchange of buffers for assays minimizes dispersion effects. This provides highly reliable results with minimal sample usage due o its pairing with extremely low low-cell volumes.

 

The heart of the system – the sensor chip

BIAffinity® uses sensor chip technology. These glass-based chips make it possible to design functional surfaces that are biocompatible and highly specific with adjustable binding capacity. Simple handling, reproducible results, minimal non-specific binding characteristics, high flexibility and regeneration stability are the hallmarks of the sensor chip.

 

The measurement principle…

is based on reflectometric interference spectroscopy (RIfS), which uses multiple reflection at thin, transparent layers. Incident light will be partially reflected at each thin-layer interface. A characteristic interference pattern (interferogram) is produced by superimposing the reflecting rays. Binding molecules to the specific surface of a sensor chip causes a change in the optical layer thickness, which in turn causes a shift of the interferogram. This makes RIfS a fast and elegant method for detecting binding events on surfaces. Reflectometric interference spectroscopy (RifS) Label-free detection principle Real-time analysis of binding events Online monitoring of interactions.

     

  • Reflectometric interference spectroscopy (RifS)
  • Label-free detection principle Real-time analysis of binding events
  • Online monitoring of interactions